Effect of fibroblast growth factor on injured periodontal ligament and cementum after tooth replantation in dogs
유상준, 이중석, 정의원, 박주철, 김병옥, 최성호,
소속 상세정보
유상준 ( Yu Sang-Joun ) - Chosun University School of Dentistry Department of Periodontology
이중석 ( Lee Jung-Seok ) - Yonsei University College of Dentistry Department of Periodontology
정의원 ( Jung Ui-Won ) - Yonsei University College of Dentistry Department of Periodontology
박주철 ( Park Joo-Cheol ) - Seoul National University School of Dentistry Department of Oral Histology
김병옥 ( Kim Byung-Ock ) - Chosun University School of Dentistry Department of Periodontology
최성호 ( Choi Seong-Ho ) - Yonsei University College of Dentistry Department of Periodontology
KMID : 0363020150450030111
Abstract
Purpose: The purpose of this animal study was to perform a histological and histomorphometric analysis in order to elucidate the effect of fibroblast growth factor-2 (FGF-2) on injured periodontal ligament (PDL) and cementum after tooth replantation in dogs.
Methods: The roots of 36 mandibular premolars from six mongrel dogs were used in this study. The roots were randomly divided into three groups: (1) a positive control group (n=12), in which the PDL was retained; (2) a negative control group (n=12), in which the PDL and the cementum between the notches were removed; and (3) an experimental group (n=12), in which the PDL and the cementum between the notches were removed and the roots were soaked in an FGF-2 solution (30 μg/0.1 mL). After treating the root surfaces, the extracted roots were replanted into extraction sockets. The animals were sacrificed four and eight weeks after surgery for histologic and histomorphometric evaluation.
Results: At four and eight weeks, normal PDLs covered the roots in the positive control group. In the negative control group, most replanted roots showed signs of replacement resorption. In the experimental group, new PDL-like tissue and cementum-like tissue were observed to partially occupy the region between the root surfaces and the newly formed bone. Histomorphometric analysis showed that the mean length of the newly formed cementum-like tissue on the roots treated with FGF-2 was significantly greater than that of the tissue on the roots in the negative control group (four weeks, P=0.008; eight weeks, P=0.042). However, no significant differences were observed between the roots treated with FGF-2 and the negative control roots with respect to newly formed PDL-like tissue.
Conclusions: The results of this study suggest that use of FGF-2 on injured root surfaces promotes cementogenesis after tooth replacement in dogs.
키워드
Cementogenesis ; Growth factor ; Periodontium ; Regeneration ; Tooth replantation
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